Incidence and antibacterial sensitivity of Salmonellae and Enterobacter hormaechei isolated from some milk products sold in Assiut City, Egypt

Document Type : Original Articles

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Abstract

The Enterobacteriaceae family includes genera of Escherichia, Salmonella, Enterobacter and Shigella, amongst others. Salmonella are the most common pathogenic bacteria in animals and humans causing Salmonellosis. While, Enterobacter hormaechei is a bacterium within the Enterobacter cloacae complex, which causes nosocomial infections. This study was carried out to detect the incidences of Salmonella spp. and Ent. hormaechei in one hundred and twenty milk products samples (thirty each of soft cheese, hard cheese, yoghurt and ice cream). The bacteriological examination revealed that Salmonella spp. failed to be detected in the examined samples, while Ent. hormaechei was isolated in 6.66% of ice cream samples, and failed to be detected in other products. Moreover, the isolated strains subjected to nucleated sequence analysis using
universal primers of16S rRNA gene to completely identify the isolates. Also, this study estimated thesensitivity of the two isolated strains to nine commercial antibiotics. In addition, the antimicrobial efflux pump activity was detected by testing the effect of two efflux pump inhibitors on the minimal inhibitory concentration of antibiotics.

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